Part:BBa_K209439:Design

pEF1-SSFYFP-hM2D-ITSN

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3656
  Illegal PstI site found at 346
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3656
  Illegal NheI site found at 1161
  Illegal PstI site found at 346
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3656
  Illegal BglII site found at 128
  Illegal BglII site found at 3776
  Illegal BamHI site found at 955
  Illegal BamHI site found at 1665
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3656
  Illegal PstI site found at 346
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3656
  Illegal PstI site found at 346
  Illegal NgoMIV site found at 2427
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 84
  

Design Notes

Cloned into pEF5/FRT/V5-DEST.